CATT/T.evansi REAGENTS (KIT R250 tests)
Kit containing 6 vials CATT antigen (2.5 ml/vial), 1 vial CATT buffer (30 ml/vial), 1 vial positive control (2.5 ml/vial) and 1 vial negative control (2.5 ml/vial). In total, 250 tests can be performed with one kit.
Storage temperature: between 2 – 8°C
Infection with Trypanosoma evansi results in production of circulating antibodies against several surface antigens of the parasite. Such antibodies can be demonstrated in the plasma or serum of the infected host by direct agglutination. The CATT-antigen is a freeze dried suspension of purified, fixed and stained bloodstream form trypanosomes expressing a predominant variable antigen type of Trypanosoma evansi (RoTat 1.2). The test is performed on a plastified card. Twenty-five microliter of diluted serum or plasma are mixed with one drop of reconstituted antigen. When antibodies are present in the test sample, trypanosomes agglutinate within 5
minutes rotation at 70 rpm.